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Forschungsvorhaben werden in den Schwerpunkten Bioverfahrenstechnik und Industrielle Biotechnologie mit öffentlichen Fördergeldgebern und Industriepartnern durchgeführt. Ausgewählte Projekte:
Im Rahmen der Förderlinie QualiFive der Hochschule München und dem BMBF-Projekt "ZUG" entstanden verschiedene neue Lehransätze, die in den Modulen "Bioverfahrenstechnik", "Verfahrens- und Umwelttechnik" sowie "Industrielle Biotechnologie" fest integriert und stetig weiterentwickelt werden.
ORCiD: 0000-0002-0543-8998
Lapierre, F., Kustermann, A. and Huber, R., Determination of nutritional requirements of calcite precipitating Sporosarcina pasteurii to increase biomass yield for biocementation. Chemie Ingenieur Technik, 92: 1230-1230. (2020) https://doi.org/10.1002/cite.202055301
Lapierre F., Schmid J., Ederer B., Ihling N., Büchs J., Huber R.
Revealing nutritional requirements of MICP-relevant Sporosarcina pasteurii DSM33 for growth improvement in chemically defined and complex media
Sci Rep 10, 22448 (2020). https://doi.org/10.1038/s41598-020-79904-9
Schmid J., Schwarz S., Fischer M., Sudhop S., Clausen-Schaumann H., Schieker M., Huber R.
A laser-cutting-based manufacturing process for the generation of three-dimensional scaffolds for tissue engineering using Polycaprolactone/Hydroxyapatite composite polymer.
Journal of Tissue Engineering, 23 July 2019, https://doi.org/10.1177/2041731419859157
Schmid J., Schwarz S., Meier-Staude R., Sudhop S., Clausen-Schaumann H., Schieker M., Huber R.
A perfusion bioreactor-system for cell seeding and oxygen-controlled cultivation of 3D-cell cultures.
Tissue Engineering Part C: Methods, 2018, Vol. 24, No. 10
Schmidberger T., Posch C., Sasse A., Gülch C., Huber R.
Forecasting product quality and quantity of mammalian cell culture processes by performance based modeling.
Biotechnol Prog. 2015 Jul-Aug;31(4):1119-27
Palmen T., Scheidle M., Huber R., Kamerke C., Wiesner A., Dittrich B., Klee D., Büchs J.
Influence of initial pH values on the lag phase of Escherichia coli and Bacillus licheniformis batch cultures.
Chemie Ingenieur Technik, 2013, 85(6), 863–871
Schmidberger T., Gutmann R., Bayer K., Kronthaler J., Huber R.
Advanced online monitoring of cell culture off-gas using proton transfer reaction mass spectrometry.
Biotechnol Prog. 2013 Dec 20. doi: 10.1002/btpr.1853
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Huber R., Roth S., Rahmen N., Büchs J.
Utilizing high-throughput experimentation to enhance specific productivity of an E.coli T7 expression system by phosphate limitation.
BMC Biotechnology 2011, 11:22
Kunze M., Huber R., Gutjahr C., Müllner S., Büchs J.
Predictive tool for recombinant protein production in E. coli shake flask cultures using an on-line monitoring system.
Biotechnology Progress, 2011, 19 Oct, DOI: 10.1002/btpr.719
Huber R., Wulfhorst H., Maksym L., Stehr R., Pöhnlein M., Jäger G., Spieß A., Büchs J.
Screening for enzyme activity in turbid suspensions with scattered light.
Biotechnol Progress, 2011 Mar/Apr, 27(2): 555-61
Huber R.
High-throughput experimentation for microscale cultivations and recombinant protein expression.
Dissertation, RWTH Aachen University, 2010
Huber R., Palmen T.G., Ryk N., Hillmer A.K., Luft K., Kensy F., Büchs J.
Replication methods and tools in high-throughput cultivation processes - recognizing potential variations of growth and product formation by on-line monitoring.
BMC Biotechnology, 2010, 10:22
Drepper T., Huber R., Heck A., Circolone F., Hillmer A.K., Büchs J., Jaeger K.E.
Flavin Mononucleotide-Based Fluorescent Reporter Proteins Outperform Green Fluorescent Protein-Like Proteins as Quantitative In Vivo Real-Time Reporters.
Appl Environ Microbiol, 2010 Sep,76(17):5990-4
Kottmeier K., Müller C., Huber R., Büchs J.
Increased product formation induced by a directed secondary substrate limitation in a batch Hansenula polymorpha culture.
Appl Microbiol Biotechnol, 2010 Mar,86(1):93-101
Huber R., Ritter D., Hering T., Hillmer A.K., Kensy F., Müller C., Wang L., Büchs J.
Robo-Lector – a novel platform for automated high-throughput cultivations in microtiter plates with high information content.
Microbial Cell Factories, 2009, 8:42
Huber R., Scheidle M., Dittrich B., Klee D., Büchs J.
Equalizing growth in high-throughput small scale cultivations via precultures operated in fed-batch mode.
Biotech Bioeng, 2009 Aug, 103(6):1095-102
Grimm R., Huber R., Neumeier T., Seidl A., Haslbeck M., Seibert FS.
A rapid method for analyzing recombinant protein inclusion bodies by mass spectrometry.
Anal Biochem. 2004 Jul 1; 330(1):140-4